LBM-Broth Miller Size:1000g 1 Suspend 16

1 Suspend 16

Dilute 50 ml MOPS-SDS 20X into 950 ml of deionised water to make 1 litre of MOPS-SDS Running Buffer

Cold Spring Harbour laboratory

Amino Acids   Arg 700 Asp 300 Cys 200 GluA 500 Gly 900 His 300 Ile 600 Leu 900 Lys 900 Met 300 Phe 500 Pro 800 Thr 500 Trp 200 Val 700 Total 8300 mg/l Please enquire about custom made formulations

21 pH (100 g/l) 5

LBM-Broth Miller Size:1000g 1 Suspend 16LBM Broth High Salt In LBM media Mg2 is added to enhance adsorption of phage lambda to the cells. Tryptone and Yeast extract do not contain enough Mg2 for optimal adsorption. Formula g l Tryptone 10 Yeast extract 5 NaCl 10 MgSO4. anhydrous 0. 98 Final pH 7. 0 0. 2 at 25C Suspend 26 gram powdered medium in 1 litre distilled water Store dry at room temperature